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DNA sequencing Assignment Help

DNA sequencing is the method where sequence of nucleotide is determined in the DNA molecule. Therefore for this method different types of method and technologies are used. Some of the basic techniques are:

  • Maxam-Gilbert method
  • Chain termination method

Maxam-Gilbert method uses radioactive labeling and the purified strand obtained from this sample is used in further cloning technology.

Chain termination method is developed by Sanger, also called Sanger method. In this method fewer chemicals and radioactive materials are used in comparison to Maxam-Gilbert method. This method is used in first generation of DNA.

DNA sequencing Assignment Help

These are only the basic techniques for DNA sequencing, but with time and advancement many other techniques has also been introduced, such as:

Shotgun sequencing: this sequencing method is used for Analysis of DNA fragments longer than 1000 base pairs.

Bridge PCR: in his case DNA colonies are formed by amplification of fragments upon primers.

Beside above mentioned techniques, some of the techniques that use low cost and thus provide effective results. Some of them are:

  • Solid sequencing
  • Illumina sequencing
  • Polony sequencing
  • MPSS
  • SMRT sequencing and many more.

All these sequencing methods have different methodology and processes, some are effective to give one result and other can be applied in different field. The above mentions are just a short and brief outlook of the topic.

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