Gel Electrophoresis Assignment Help
Are you confused on the steps and process of electrophoresis?
Electrophoresis is one of the scientific techniques for the separation of molecules in mixtures to obtain them in a pure form and when gel is used for this process, it is called gel electrophoresis. This technique is generally used in estimation of types and size of molecules like the protein and nucleic acid. The molecules thus obtain by this process are used for further inspections and research activities. Generally two types of gel are used for this process as per the types and need of separation. The two gels used are:
- Agars
- PAGE called polyacrylamide gel electrophoresis.
If we first take agarose, it is made from sea weeds thus, consists of long polysaccharides chain forming matrix of large pores. It is used for separation of large DNA molecules whereas PAGE have smaller pores and are used to separate smaller DNA molecules and is made of covalent bonding of acrylamid and bis- acrylmaid. One of the main difference between PAGE and Agarose are PAGE is prepared chemically whereas Agarose occurs naturally.
Therefore, though the types of gel varies but use of correct gel as per the molecules that are to be separated is important because wrong choice of gel can restrict the overall process of separation.
Gel electrophoresis is done by the application of electric field on the matrix. Due to applied electricity, molecules separate and thus, smaller molecules travel faster and larger molecules remains behind.
This is just a short and very brief description about gel electrophoresis, but there are still small details yet very important that can help students cover the complete topic excellently.
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